منابع مشابه
The Journal of Biological Chemistry
Human serum albumin (HSA) has been allowed to react with varying amounts of iodine under mild conditions. Almost all of the tyrosyl residues of HSA may be converted to iodotyrosyl residues (primarily diiodotyrosyl) without producing significant changes in the conformation of the protein. Difference spectral measurements in urea show the disappearance of “burled” tyrosyl and the appearance of “b...
متن کاملThe Journal of Biological Chemistry
An aminopeptidase from Escherichia coli has been purified to homogeneity and crystallized. This peptidase is responsible for about 65% of the hydrolytic activity found in crude extracts toward the substrate methionylalanylserine. It resembles the so-called leucine arninop!ptidase of hog kidney in several respects, including size, metal requirements, and peptide specificity. Evidence based on su...
متن کاملThe Journal of Biological Chemistry
1. Highly purified arginineand lysine-rich histone was maleylated and then hydrolyzed with trypsin. A large amount of precipitate approximating 35% of the histone appeared during the demaleylation procedure. The precipitate was separated into four fractions on a Sephadex G-50 column and one fraction, tryptic peptide II from the maleylated histone, was found to be the COOH-terminal portion of th...
متن کاملThe Journal of Biological Chemistry
A very rapid procedure for the preparation of phosphoglycerate mutase from chicken breast muscle is presented. Commercially available frozen chicken breasts may be used. The enzyme is obtained in high yields, and the specific activity of the crystalline homogenous preparations is equal to that of the crystalline enzymes from yeast and rabbit muscle. The chicken breast enzyme requires 2,3-diphos...
متن کاملThe Journal of Biological Chemistry
A two-phase system in vitro has been developed for studying the bone-mobilizing effects of parathyroid hormone. Phase I consists of the incubation of hormone with ascites cells, and Phase II is the incubation of Phase I supernatant with dead bone powder. The mobilization of labeled calcium from this powder is the index of hormonal activity. The response to hormone is apparent at lo-* M hormone,...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2021
ISSN: ['1083-351X', '0021-9258', '1067-8816']
DOI: https://doi.org/10.1074/jbc